Semiotics is both a rich theoretical framework and a versatile method of analysis, appearing across numerous academic disciplines—from film and media studies to the emerging field of biosemiotics. This library research guide introduces the foundational concepts of semiotic theory, offering accessible pathways into its key ideas, terminology, and analytical tools. With a particular emphasis on applications within the humanities, it is designed to support scholarship and teaching. Students and educators in philosophy, literature, film, communication, media, music, and visual arts will discover an engaging starting point for deeper research.